[ study of antibacterial effect of humulus lupulus on some of gram positive and gram negative bacteria]

In recent years, a new view has been stablished toward pharmaceutical plants and applicational use of them all over the world and researchers, in various medical courses are investigating about this issue. On this basis, antibacterial effect of Humulus lupulus, the pharmaceutical plant which has an important place in traditional medicine of Iran, was evaluated on some of bacteria. Based on scientific methods after being dried, prepared extract by using macerasion method. Antibacterial effects of ethanol extract were studied on Staphylococcus aureus [1112PTCC], Escherichia coli [1276PTCC], Bacillus subtilis [1023PTCC], Psedomonas aeroginosa [1430PTCC], by using spreading method, well plate method and MIC. The ethanol extract of Humulus lupulus showed antimicrobial activity on S. aureus and B. subtilis. The extract exhibited antimicrobial activity at a range of 125 mg/ml to 62/5 mg/ml against S. aureus and B. subtilis respectively. With the increase of worldwide antibiotic resistance of many strains of disease producing bacteria, there is a need to access new and complementary approaches to antibiotic therapy. Extract of Humulus lupulus has antibacterial effect. The antimicrobial activities of Humulus lupulus against a variety of gram positive bacteria confirm its use for treatment of infectious

[ study of anti-microbial effect of pycnocycla spinosa's fruit extracts]

Infectious diseases account for approximately one-half of all deaths in tropical country. In developed country, despite the progress made on the control of disease, incidence of epidemics due to drug resistant microorganisms and unknown diseases spreading microbes pose enormous public health concerns. On the other hand, in spite of improvements in food production hygiene, food safety is increasingly an important health issue. There is, therefore, still a need for new antimicrobial agent to reduce or eliminate foodborne pathogen as well as food spoilage microorganisms. Historically, plants play a major role in primary health-care as therapeutic remedies in developing countries. The screening of plant extracts has been of great interest to scientists for the discovery of new drugs effective in the treatment of infectious disease. Umbelleferea is known to be a potential source for the antimicrobial agents. The present study attempts to investigate the antimicrobial activity of Pycnocycla spinosa as a member of Umbelleferea against selected microorganisms. The plants were collected from Isfahan and different solvent extracts of plants were prepared. Then, the antimicrobial activity of extract was determined, using disk diffusion method. The minimal inhibitory concentration [MIC] was determined by tube dilution method. Results demonstrated that different extracts of plants indicate antimicrobial activity against bacills subtilis, aspergilus niger and candida albicans. Generally, the antimicrobial activity of the plant's fruit extracts is considered medium. Pycnocycla spinosa fruit's extract showed medium antimicrobial activity. Hydroalcoholic extract of the fruit demonstrated higher antimicrobial activity. This may reflect a low concentration of active components in extracts. Further studies are needed to investigate antimicrobial activity of the plant's essential oil and other parts of the plant

[Quality and quantity evaluation of flavonoids in the aerial parts, flowers and fruits of Varthemia persica [var. persica] DC]

Varthemia persica is an aromatic plant belonging to the Astraceae family. The plant is endemic to Iran. It contains volatile oils and the main constituents of volatile oils are sesquiterepens. There is no information regarding flavonoid constituents. Extract was prepared from freeze-dried power of flower, fruit and aerial parts with methanol-water [1-1] and HCl [1.2 M]. Determination of myricetin, Lute Olin, apigenin, quercetin and kaempferol in all samples were carried out by HPLC. The plant flowers contain all five mentioned flavonoids, however, fruit and aerial parts contain only kaempferol, lute Olin and quercetin. The plants can be considered as flavonoid containing plant and are a good candidate for pharmacological studies